Australian researchers investigated the combined effects of phytosterols and (n-3) long-chain polyunsaturated fatty acids (LCPUFA) on plasma lipid profile in hyperlipidaemic individuals. This study was a 3-week, double-blind, placebo-controlled, 2 × 2 factorial RCT in four parallel groups of 60 hyperlipidaemic individuals. Subjects were randomised to receive either sunola oil or 1.4 g/day (n-3) LCPUFA capsules with or without 2 g phytosterols per day while maintaining their habitual diet. The combination of phytosterols and (n-3) LCPUFA reduced plasma total cholesterol by 13.3%, which differed from (n-3) LCPUFA alone. LDL-C concentrations followed the same pattern as that of plasma cholesterol with a 12.5% decrease in the combination group. The HDL-C concentration was increased by (n-3) LCPUFA (7.1%) alone and in combination with phytosterols (8.6%), whereas phytosterol treatment alone had no effect. Plasma triglyceride concentration was lowered by (n-3) LCPUFA (22.3%) alone and in combination with phytosterols (25.9%), whereas phytosterol treatment alone had no effect.