In this pilot clinical study, acupuncture was incorporated into the standard care for adult patients with osteoarthritis of the knee to determine its contribution to pain relief and improved mobility. In a prospective, non-randomised study, patients with osteoarthritis of the knee were separated into two groups. The first (the experimental group; n = 12) was scheduled for up to eight sessions of acupuncture in addition to standard care, while the second (the control group; n = 12) received standard care only. Measurements using the 6-min walking distance test, pain VAS, and osteoarthritis of the knee outcome measurement were taken at baseline and after 4 weeks. Both study and control groups showed significant improvement with respect to time effects in terms of 6-min walking distance, pain VAS, pain domain and mobility domain scores determined by the osteoarthritis of the knee outcome measurement, after adjusting for co-variables. However, improvements measured in the study group did not differ significantly from those in the control group.